Application of immunoperoxidase (PAP) technique for demonstration of deposited immunoglobulins in renal biopsies.

The presence of different immunoglobulins (lgG, IgM, and IgA) was studied in 50 routinely fixed paraffin embedded renal biopsies. lgG deposits was seen independently in 26 (52%) cases, in combination with IgA in 14 (28%) cases, with IgM in 1 (2%) case and with both lgA/lgM in 1 (2%) case. lgA alone was seen in 1 (2%) case only. IgM was seen only in 2 (4%) cases. In one case it was seen in combination with IgG and in the other case with both lgG & IgM. The PAP staining technique was found to be extremely useful, It has many advantages over the established Immunofluorescence technique including lower cost and less cumbersome methodology. It was found that PAP stain along with methenamine silver stain was extremely useful in histopathological classification.(JPMA: 38 : 66 , 1988).